CURRICULUM VITAE

 

          Jianxun Yi           

           

EDUCATION:

 

            1980-1985:    Medical student

                                    Faculty of Medicine

                                    Hunan Medical University, Changsha, P. R. China

                                    M.D. granted.

                                     

            1988-1991:    Graduate  student 

                                    Department of Molecular Biology

                                    Hunan Medical University

                                    Changsha, Hunan

                                    P. R. China

                                   

 

PROFESSIONAL EXPERIENCE:

           

            Oct, 2000 – Present: Research assistant

                                 Dr. Eduardo Rios’ lab          

                                 Department of Molecular Physiology and Biophysics

                                 Rush University School of Medicine

                                 1750 W. Harrison

                                 Chicago, IL 60612

 

            July, 1998 – Oct, 2000:   Research assistant

                                    Dr. Kathy Murray’s lab     

                                    Clinical Pharmacology

                                    Vanderbilt University, School of Medicine

                                    23rd Ave @ Pierce, MRB II 559

                                    Nashville, TN 37232

 

            July, 1995 - July, 1998:   Research assistant

                                    Dr. E. Rios and R. Shirokov’ s Lab

                                    Department of Molecular Physiology and Biophysics

                                    Rush University School of Medicine

                                    1750 W. Harrison

                                    Chicago, IL 60612

           


           

 

            Jan, 1994 - July, 1995:  Research Assistant

                                    Dr. J. Ma’s Lab

                                   Department of Physiology and Biophysics

                                   School of Medicine

                                    Case Western Reserve University

                                    Cleveland, OH

 

           

            Aug, 1988 - Aug, 1991:  Research Assistant (Graduate Student)

                                    Department of Molecular Biology

                                    Hunan Medical University

                                    Changsha, P.R. China.

 

            Aug, 1985 - Aug, 1988:  Teaching and Research Assistant

                                    Department of Histology and Embryology

                                    Hunan Medical University

                                    Changsha, Hunan

                                    P. R. China

 

 

RESEARCH TECHNIQUES:

 

            DNA and RNA isolation;  mRNA purification; 

            primer design, RT-PCR, PCR; 

            cDNA cloning;  site-directed mutation, chimera construction, cDNA sequencing;

            mammalian cell culture and transfection;

            Western, Northern and Southern blot;

            Primary culture of myoblasts.

 

 

PUBLICATIONS:

           

1.  Zhou, J., Shin, HG, Yi, J., Shen, W, Williams CM, and Murray KT. (2001). Phosphorylation and ER retention signals mediate trafficking of the human cardiac sodium channel by protein kinase A.  Circ. Res.  In review.

 

            2. Zhou, J., Yi, J., Hu, N., George Jr. AL., and Murray, KT. (2000) Activation of protein kinase A modulates trafficking of the human cardiac sodium channel in Xenopus oocytes.  Circ. Res. 87: 33-38. 

 

            3.  Shirokov, R., Ferreira, G., Yi, J. and Rios, E.  (1998) Inactivation of gating currents of L-type calcium channels.  Specific role of the "2* subunit.  J. Gen. Physiol. 111: 807-823.

 

            4. Zhou, J., Cribbs, L., Yi, J., Shirokov, R., Perez-Reyes, E. and Rios, E. (1998) Molecular cloning and functional expression of a skeletal muscle dihydropyridine receptor from Rana catesbeiana. J. Bio. Chem. 273(39): 25503-25509.

 

            5. Gonzalo, F., Yi, J., Rios, E. and Shirokov, R.  (1997)  Ion-dependent inactivation of barium current through L-type calcium channels.  J. Gen. Physiol.  109: 449-461.

 

 

ABSTRACTS:

 

            1. Zhou J, Gonzalez A, Segura R, Rios E, Ferreira G, Yi J, Brum G. (2002). Modulation by Ca2+ and Mg2+ of mammalian muscle Ca2+ sparks. Biophys. J.  82 (1) :  2490.2.

 

2. Zhou J, Shin HG, Yi J, Shen W, and Murray KY.  (2001).  Structural requirements in the human cardiac sodium channel for regulated trafficking by protein kinase A.  Circulation.   (Abstract accepted for an oral presentation at Scientific Sessions 2001, in Anaheim, CA., American Heart Association.)

 

3.  Zhou, J., Yi,  J., Hu, N., George, A., and Murray, K.T. (2000). Activation of protein kinase A modulates trafficking of the human cardiac Na2+ channel: Role of Serines 525 and 528 in the I-II interdomain loop. Circulation. 102(18): II-263.

 

            4. Chien, A., Yi, J. and Shirokov, R.  (1998)  The "2 domain affects inactivation of gating currents in Ca2+ channels. Biophys. J.  74 (2) :  A101.

 

            5. Zhou, J.,  Cribbs, L.,  Yi, J.,  Shirokov, R.,  Perez-Reyes, E.  and Rios, E.  (1997)  Cloning of an L-type Ca channel homolog from frog skeletal muscle and functionally expression of a chimeric channel.  Biophys. J.  72:  A146.

 

            6. Ferreira, G., Yi, J., Rios, E. and Shirokov, R.  (1997)  Barium-dependent inactivation of L-type calcium channels.  Biophys. J.  70: A112.

 

            7. Shirokov, R., Ferreira, G., Yi, J., and Rios, E.  (1997)  Effect of the "2* subunit on voltage-dependent inactivation of cardiac calcium channels.    Biophys. J.  70: A112.

 

            8. Ma, J., Drumm, M.L., Zhao, J., Xie, J., Yi, J. and Davis, P.  (1997)  The R domain is required for the normal function of CFTR chloride channel.  Biophys. J.  70: A113.

 

            9. Shirokov, R., Ferreira, G., Yi, J., Zhou, J. ,  Chien, A., Hosey, M.,  and Rios, E.  (1996)  Similarity of biophysical properties of Ca currents in "1C and "1C/$2A transfected mammalian cells.  Biophys. J.  70: A183.

 

            10. Yi, J., Xie, J., Davis, P.B., and Ma, J.  (1996)  Mutation of proline residues in the R domain leads to increase in open probability of the CFTR chloride channel.  (Abstract) For  “ Cystic Fibrosis Foundation”,  Tenth Annual North America of Conference, Orlando, FL, Oct. 24-27, 1996.