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Photo:  November, 2004


Eduardo Ríos CV

 

Personal Data:

Eduardo Ríos. Born May 8, 1947, in Tacuarembó, Uruguay.

Married in 1970 to Matilde Musulén. Three daughters (Lara, Marina and Carmela)

US citizen since 1993.

 

Degrees:

B.Sc.,  Tacuarembó, Uruguay, 12/28/63

"Licenciado" (Physics), School of Sciences, Montevideo, 6/28/75

 

Higher Education:

School of Medicine, Montevideo (3/1/64 - 8/1/67).

School of Engineering, Montevideo (3/1/68 - 12/28/70).

School of Sciences, Institute of Physics, Montevideo (3/1/71 - 6/28/75).

School of Medicine, University of Rochester, New York (3/1/78 - 6/28/83).

 

Thesis:

"Physicochemical Models of Ionic Movements in Excitation-Contraction Coupling" (in Spanish). School of Sciences, Montevideo, 1975.

 

Awards and Academic Services:

Research Award of the University of Montevideo, years 1967-1970.

UNESCO Fellowship, 1976.

Member, Physiology Study Section, NIH, 1989-1993.

Member, National Reviewer's Reserve, 1993 to date.

Member, Cardiovascular Science Study Section, NIH, 2001..

Vice-Chair, Gordon Conference on Muscle, 1994.

Chair, Gordon Conference on Muscle, 1997.

Research Council. American Heart Association of Metropolitan Chicago. 1992-1996.

Council Member, Society of General Physiologists, 1995-1997.

Founder, Society of Latin American Biophysicists, and past member of its Council and Executive Group. 

Member, Advisory Committee on Muscle Research. National Institute of Arthritis and Musculoskeletal and Skin Diseases. National Institutes of Health. 1996.

M.E.R.I.T. (Method to Extend Research in Time) Award of the National Institute of Arthritis and Musculoskeletal and Skin Diseases, 1996-2001.

Council Member, National Institute of Arthritis and Musculoskeletal and Skin Diseases, 1998-2001.

GPRA Evaluator of National Institutes of Health. 2000.

M.E.R.I.T. Award Extension, 2001-2006.

International advisor, Editorial Board of the Japanese Journal of Physiology.

Council Member, The Biophysical Society, 2003-2005.

Chair, Journal of Physiology Symposium, “Calsequestrin, triadin and more.  The proteins that modulate calcium release in cardiac and skeletal muscle”.  Boston, 2009.

Mentorship Award.  Rush University, 2013.

Associate Editor, The Journal of General Physiology; August 2014 to date.

 

Editorial Boards:


The Journal of General Physiology. 1992 to 2014.

The American Journal of Physiology (Cell). 1994 to 2005.

The Japanese Journal of Physiology. 2000 to date.

Physiological Reviews. 2000 to 2006.

The Journal of Physiology, London.  2004-2010

 

Consejero Editorial (Editorial Advisor) , Anales de la Facultad de Medicina (Montevideo).  2013 to date. 

 

Referral and Editorial Work:


The Journal of General Physiology, The Biophysical Journal, Nature, Nature Medicine, Science, The Journal of Muscle Research and Cell Motility, Proceedings of the National Academy of Sciences (USA), Molecular Pharmacology, Pflügers Archiv, The Journal of Physiology, The American Journal of Physiology, The Japanese Journal of Physiology, Physiological Reviews, News in Physiological Sciences, The Journal of Cell Biology, the Journal of Biological Chemistry, the EMBO Journal, FEBS Letters, Sístole, the Journal of Membrane Biology. ACS Chemistry & Biology. PLOS One. 

 

Member of the Physiology and the Cardiovascular Sciences (CV1) Study Sections of N.I.H. Referrals for the National Science Foundation, American Heart Association, Muscular Dystrophy Association, Myositis Foundation, U.N.E.S.C.O. the Uruguayan Program of Development of the Basic Sciences (PEDECIBA) and National Council of Science and Technology (CONICYT) of Chile, Argentina and Uruguay. The Agencia Nacional de Investigación e Información (ANII) and the Comisión Sectorial de Investigación (CSIC) of Uruguay, the Canadian Research Council, the Australian Research Council, the Consiglio Nazionale Della Ricerca and the Telethon Foundation (Italy) and the Austrian National Research Council.

 


Positions:

Post-Doctoral:

Director, Section of Cellular Signaling, Rush University School of Medicine, Chicago (6/1/03 – present).

Professor, Dept. of Molecular Biophysics and Physiology, Rush University School of Medicine, Chicago (7/1/91 - present).

Associate Professor, Dept. of Physiology, Rush University School of Medicine, Chicago (7/1/87 - 6/30/91).


Assistant Professor, Dept. of Physiology, Rush University School of Medicine, Chicago (7/1/83 - 7/1/87).

Professor,  PEDECIBA (National Program of Development of Basic Sciences). Dept. Biophysics School of Medicine, Montevideo, Uruguay (honorary). 1995 to date.

Assistant Professor, Dept. of Physiology, School of Medicine, University of Rochester, Rochester, NY (7/1/82 - 7/1/83).

Assistant Professor, Dept. of Biophysics & Biochemistry, School of Dentistry, Montevideo (9/1/75 - 12/30/82).

Research Associate, Dept. of Physiology, School of Medicine, University of Rochester, Rochester, NY (12/13/78 - 6/18/79, and 6/3/80 - 6/30/82).

Research Associate, Dept. of Biophysics, "Clemente Estable" Institute for Biological Sciences, Montevideo (8/1/75 - present; honorary, tenured).

 

Pre-Doctoral:

Research Fellow, Dept. of Biophysics, School of Medicine, Montevideo, Uruguay (3/1/67 - 3/1/70).

Instructor, Department of Physics, School of Engineering, Montevideo, Uruguay (3/1/71 - 3/1/72).

Instructor of Biophysics, Dept. of Biophysics, School of Medicine, Montevideo, Uruguay (3/15/73 - 2/1/76).

 

University Services:

Member of the University Cloister in Montevideo, Uruguay. This is an elective body that performs the legislative tasks in the university government, 1966 - 1970.

Task force on Population and Family Planning, Montevideo, 1970.

Task force on University Reform, Montevideo, 1971.


Standing Committee on Affirmative Action, Rush University, 1984-1986.

Task force on Quality of Education, Rush University, 1986-1988.

National Program of Development of Basic Sciences, Montevideo, Uruguay, May 1988 - present.

Task force on Research, Rush University, 1991-92.


Council Member, American Heart Association of Metropolitan Chicago, 1992 to 97.

Institutional Animal Use and Care Committee. Rush University 1996-1998.

Committee on Committees. Rush University. 1995- 2013.

Rush University Department of Molecular Biophysics and Physiology Chair Search Committee.  2015.

Rush University Department of Molecular Biophysics and Physiology Advisory Committee. 2000 to date.

Academic Affiliations:

The Biophysical Society.

The Physiological Society.

Sociedad Uruguaya de Ciencias Fisiológicas.

The Society of General Physiologists.

The American Physiological Society.

Society of Latin American Biophysicists (SOBLA).


Research Support, current or recently completed:

Roles of calsequestrin in the control of calcium signals in health and disease”. Grant from the National Institute of General Medical Sciences.  Current funding period: June 2014 – May 2018. This is a multi-center grant; Principal Investigators: E. Rios and C. Kang (Washington State University).

“Skeletal Muscle.  Ca regulation inside the Sarcoplasmic Reticulum”.  Grant from the National Institute of Arthritis, and Musculoskeletal and Skin Diseases.  Current funding period: April 2009-March 2014.  Principal Investigator: E. Rios.

“Calcium Movements in Excitation-Contraction Coupling".  Grant from the National Institute of Arthritis, and Musculoskeletal and Skin Diseases.  Current funding period: July 2007 to June 2012.    Principal Investigator: E. Rios.

“A dual-scanner confocal microscope".  Shared Instrumentation Grant from the National Center for Research Resources, NIH.   P.I.  E. Rios. Funding period: March 2008 through Feb 2009.  This award is specifically for the acquisition of a LIVE DUO dual scanner (Carl Zeiss).

“A dual-scanner confocal microscope”.  Rush University Capital Equipment Hasterlik Grant.  P.I. E. Rios.  This is a matching award to the Nat’l Center grant listed above.

"Calcium Movements in Excitation-Contraction Coupling". Grant from the National Institute of Arthritis, and Musculoskeletal and Skin Diseases.  Implemented as a 10-year MERIT (Method to Extend Research In Time) award.  Funding period: July 1996 to June 2007.

Trainees:

1. Gustavo Brum. Postdoctoral, 1984 - 1986. M.D. School of Medicine, Montevideo. Now the Chair of the Department of Biophysics, School of Medicine at the Universidad de la República, Montevideo.

2. Robert Fitts. Sabbatical trainee under NRSA, Public Health Service, 1986. Professor, Department of Biology, Marquette University, Milwaukee.

3. Michael Fill. Postdoctoral, part of 1986. Ph.D. University of Illinois at Champaign, Urbana. Now a Professor at Rush University, Molecular Biophysics and Physiology.

4. Laszlo Csernoch. Postdoctoral, 1988. Ph.D. University Medical School of Debrecen. Now the Chair in the Department of Physiology, Debrecen University, Hungary.

5. Gonzalo Pizarro. Postdoctoral, 1986 - 1988. M.D. School of Medicine, Montevideo. Now an Associate Professor in the Department of Biophysics, School of Medicine, Montevideo.

6. Ismael Uribe. Postdoctoral, 1987 - 1988. M.D., Ph.D. Inst. Politécnico Nacional. Now an Assistant Professor at Universidad de Chihuahua, Mexico.

7. Adom González. Postdoctoral, 1989 - 1993. Ph.D. IVIC, Caracas, Venezuela. Then a Research Associate at Colorado State University, Fort Collins.

8. Zoltán Papp. Visiting Scientist, 1989. M.D. University of Debrecen, Hungary. Now a Assistant Professor in the Department of Computer Science, Debrecen University.

9. Jianjie Ma. Postdoctoral, 1989 - 1992. Ph.D. Baylor College of Medicine. Department of Physiology and Molecular Biophysics. Now a Professor of Physiology and Biochemistry at Ohio State University College of Medicine, Columbus, Ohio.

10. Ivan Stavrovsky. Postdoctoral, 1990. M.S. in Engineering, School of Engineering, Bratislava, Chekoslovakia. Now a Quality Manager at Siemens CMT, Bratislava and Munich.

12. Roman Skirokov. Postdoctoral, 1990 - 1992. Ph.D. in Physics. School of Sciences, Kiev, USSR. Now an Associate Professor at Rutgers, New Jersey Medical School.

13. Miloslav Karhánek. Postdoctoral, 1991. M.S. in Engineering, School of Engineering, Bratislava, Chekoslovakia. Now in the Biosensor and Biotech Group of the University of Santa Clara Genome Tech Center.

14. Natalia Shirokova. Postdoctoral, 1991-present. - Ph.D. in Biophysics, School of Sciences, Kiev, Ukraine. Now an Associate Professor at Rutgers, New Jersey Medical School.

15. Alexander Tsugorka. Postdoctoral, 1994-1997. Ph.D. in Biophysics, School of Sciences, Kiev, Urkraine.

16. Jingsong Zhou. Graduate Student. Graduated in August, 1997. Now a Professor at Kansas City University of Medicine and Biosciences.

17. Gonzalo Ferreira. Postdoctoral, 1995-1999. M.D. Universidad de la Republica, Montevideo, Uruguay. M.Sc. Universidad de la Republica. Now an Associate Professor at Universidad de la Republica.

18. Wolfgang Kirsch. Postdoctoral, 1997-2000. M.Sc. in Physics, Ruprecht-Karls University of Heidelberg, Germany. Ph.D. in Biophysics.

19. Juliana Rengifo . Graduate, 1999. B. Sc. , M. Sc. Universidad del Valle, Cali, Colombia. Now at Universidad ICESI, Cali, Colombia.

20. Rolando Segura. Graduate, 2000-present. B. Sc. , M. Sc. Universidad de la Habana. Cuba.

21. Bradley Launikonis. Postdoctoral, 2001 - 2006. BSc(Hons), PhD, La Trobe University, Melbourne, Australia.  Now a Lecturer at University of Queensland, Brisbane, Australia.

22. Demetrio Santiago. Graduate Student, 2002-2012.  Lic. Universidad Autonoma de Madrid.  Now at Katholieke Universitet, Leuven, Belgium.

 

23. Leandro Royer. Graduate Student, 2003-2009. M.Sc. Chemistry, Universidade de Sao Paulo.

 

24. Jerry Curran. Graduate Student, 2004-2010. M.Sc. University of Illinois at Chicago.  Now at Ohio State University Medical College.

 

25. Yan Li.  Graduate Student, M.Sc.  2012-2013.  University of Illinois at Chicago.

 

26.    Sandrine Pouvreau.  Postdoctoral.  2006.  Ph.D. Université de Lyon. Since February 2008: Chargé de Recherche, Conseil National de la Recherche Scientifique, Villeurbanne, France.

 

27.       Monika Sztretye.  Postdoctoral.  2007-2012.  Ph.D. Medical University of Debrecen, Hungary.  Now a Research Associate, Department of Physiology at U of Debrecen.

 

28.        Carlo Manno.  Postdoctoral. 2009.  Ph.D. Instituto Venezolano de Investigaciones Cientificas.

 

29.        Carolina Figueroa.  Postdoctoral. 2009. Ph.D. Instituto Venezolano de Investigaciones Cientificas.

 

 

Published Work:

Refereed Papers


Castro, J., E. Ríos and C. García. Characterization of the "Inotropic Effect" produced on a systole by the preceeding ones (in Spanish). Sístole, Montevideo 26: 36-47, 1975.

Ríos, E., J. Castro and C. García. The inotropic memory of amphibian myocardium. I. Identification of two simultaneous mechanisms and statement of a model. Arch. Int. Physiol. Biochim. 83: 27-43, 1975.

García, C., E. Ríos, A. Sánchez, E. Alvarez and E. Barrios. The inotropic memory of amphibian myocardium. II. Quantitative verification of a model. Arch. Int. Physiol. Biochim. 85: 435-453, 1977.

Kovács, L. E. Ríos and M.F. Schneider. Calcium transients and intramembrane charge movement in skeletal muscle fibres. Nature 279: 391-396, 1979.

Schneider, M.F., E. Ríos, and L. Kovács. Calcium transients and intramembrane charge movement in skeletal muscle. In: The Regulation of Muscle Contraction: Excitation-Contraction Coupling, A.D. Grinnell and M.A.B. Brazier, eds. New York: Academic Press, 1980.

Ríos, E. and M.F. Schneider. Stoichiometry of the reactions of calcium with the metallochromic indicator dyes Antipyrylazo III and Arsenazo III. Biophys. J., 36: 601-621, 1981.

Kovács, L., E. Ríos and M.F. Schneider. Measurement and modification of free calcium transients in frog skeletal muscle fibres by a metallochromic indicator dye. J. Physiol. (London) 343: 161-196, 1983.

Melzer, W., E. Ríos and M.F. Schneider. Time course of calcium release and removal in skeletal muscle fibers. Biophys. J., 45: 637-641, 1984.

Schneider, M.F., E. Ríos and W. Melzer. Use of a metallochromic indicator to study intracellular calcium movements in skeletal muscle. Cell Calcium 6: 109-118, 1985.

Melzer, W., E. Ríos and Schneider, M.F. The removal of myoplasmic free calcium following calcium release in frog skeletal muscle. J. Physiol. (London) 373:481-512, 1986.

Ríos, E. and Brum, G. Involvement of dihydropyridine receptors in excitation- contraction coupling in skeletal muscle. Nature, 325: 717-720, 1987.

Brum, G. and Ríos, E. Intramembrane charge movement in frog skeletal mining the rate of calcium release from the sarcoplasmic reticulum in muscle fibers. Biophys. J., 51: 1005-1007, 1987.

Brum, G., Ríos, E. and Stéfani, E. (with an appendix by Brum, G., Ríos E. and Schneider, M.F.). Effects of extracellular calcium on the calcium movements of excitation-contraction coupling in skeletal muscle fibres. J. Physiol. 398: 475-505, 1988.

Brum, G., Fitts, R., Pizarro, G. and Ríos, E. Mobile charges of the skeletal muscle membrane require calcium to function as voltage sensors of excitation-contraction coupling. J. Physiol. 398: 441-473, 1988.

Pizarro, G., Fitts, R., Uribe, I. and Ríos, E. The voltage sensor of excitation- contraction coupling in skeletal muscle. Ion-dependence and selectivity. J. Gen. Physiol. 94: 405-428, 1989.

Bean, B.P. and Ríos, E. Non-linear charge movement in the membranes of mammalian cardiac ventricular cells. J. Gen. Physiol. 94: 65-93, 1989.

Csernoch, L., G. Pizarro , I. Uribe, M. Rodríguez and E. Ríos. Interfering with Ca release suppresses I, the delayed component of intramembrane charge movement in skeletal muscle. J. Gen. Physiol. 97: 845-884, 1991.

García, J., E. Ríos and E. Stéfani. Effects of the calcium buffer EGTA on the delayed charge of skeletal muscle. J. Gen. Physiol. 97: 885-896, 1991.

Pizarro, G., L. Csernoch, I. Uribe, M. Rodriguez and E. Ríos. The relationship between Q and Ca release from the sarcoplasmic reticulum in skeletal muscle. J. Gen. Physiol. 97: 913-947, 1991.

Ma, J., Mundiña-Weilenmann, C., Hosey, M. and Ríos, E. Dihydropyridine-sensitive skeletal muscle Ca channels in polarized bilayers. I. Kinetics and voltage dependence of gating. Biophys. J. 60: 890-901, 1991.

Mundiña-Weilenmann, C., Ma, J., Ríos, E. and Hosey, M. Dihydropyridine-sensitive skeletal muscle Ca channels in polarized bilayers. II. Effects of phosphorylation by cAMP-dependent protein kinase. Biophys. J. 60: 902-909, 1991.

Ma, J., Gutiérrez, L., Hosey, M. and Ríos, E. Dihydropyridine-sensitive skeletal muscle Ca channels in polarized bilayers. III. Effects of phosphorylation by protein kinase C. Biophys. J. 63: 639-647, 1992.

Pizarro, G., Csernoch, L. and Ríos, E. Differential effects of tetracaine on two kinetic components of calcium release in frog skeletal muscle fibres. J. Physiol. 457: 525-538, 1992.

Shirokov, R., Levis, R., Shirokova, N. and Ríos, E. Two classes of gaing current from L-type a channels in guinea pig ventricular myocytes. J. Gen. Physiol. 99: 863-895, 1992.

González, A. and Ríos, E. Perchlorate enhances transmission in skeletal muscle excitation-contraction coupling. J. Gen. Physiol. 102: 373-421, 1993.

Ma, J., Anderson, K., Shirokov, R., Levis, R., González, A., Karhanek, M., Hosey, M.M., Meissner, G. and Ríos, E. Effects of perchlorate on the molecules of excitation-contraction coupling of skeletal and cardiac muscle. J. Gen. Physiol. 102: 423-448, 1993.

Rios, E., Karhanek, M., Ma, J. and González, A. An allosteric model of the molecular interactions of excitation-contraction coupling in skeletal muscle. J. Gen. Physiol. 102: 449-481, 1993.

Shirokov, R., Levis, R., Shirokova, N. and Ríos, E. Ca2+-dependent inactivation of cardiac L-type Ca2+ channels does not affect their voltage sensor. J. Gen. Physiol. 102: 1005-1030, 1993.

Shirokova, N., Pizarro, G. and Ríos, E. A damped oscillation in the intramembranous charge movement and calcium release flux of frog skeletal muscle fibers. J. Gen. Physiol. 104: 449-477, 1994.

Shirokova, N., González, A., Ma, J., Shirokov, R. and Ríos, E. Properties and roles of an intramembranous charge mobilized at high voltages in skeletal muscle. J. Physiol. 486: 385-400, 1995.

Tsugorka, A., Ríos, E., and Blatter, L.A. Imaging elementary events of calcium release in skeletal muscle cells. Science 269:1723-1726, 1995.

Chien, A.J., Zhao, X., Shirokov, R.E., Puri, T.S., Chang, C.F., Sun, D., Ríos, E., and Hosey, M. Roles of a Membrane-localized Subunit in the Formation and Targeting of Functional L-type Ca2+ Channel. J. Biol. Chem. 270:30036-30044, 1995.

Shirokova, N., García, J., Pizarro, G. and Ríos, E. Ca2+ release from the sarcoplasmic reticulum compared in amphibian and mammailian skeletal muscle. J. Gen. Physiol. 107:1-18, 1996.

Shirokova, N. and Ríos, E. Caffeine enhances intramembranous charge movement in frog skeletal muscle by increasing cytoplasmic Ca2+ concentration. J. Physiol. 493:341-356, 1996.

Shirokova, N. and Ríos, E. Activation of Ca2+ release by caffeine and voltage in frog skeletal muscle. J. Physiol. 493:317-339, 1996.

J. Chien, K.M. Carr , R.E. Shirokov, E. Rios and M.M. Hosey Identification of Palmitoylation Sites within the L-type Calcium Channel 2a Subunit and Effects on Channel Function. J. Biol. Chem. 271 26465-26468, 1996 .

Meissner, G., Ríos, E., Tripathy, A., and Pasek, D.A. Regulation of skeletal muscle Ca2+ release channel (ryanodine receptor) by Ca2+ and monovalent cations and anions. J. Biol. Chem., 272:1628-1638, 1997.

Ríos, E. and Stern, M. Calcium in Close Quarters: Microdomain feedback in excitation-contraction coupling and other cell biological phenomena. Ann. Rev. Biophys. Biomolec. Struct.,26:47-82, 1997.

Blatter, L.A., Hüser, J and Ríos, E. Sarcoplasmic reticulum Ca2+ release flux underlying Ca2+ sparks in cardiac muscle. PNAS 94: 4176-4181, 1997.

Pizarro, G., Shirokova, N., Tsugorka, A. and Ríos, E. "Quantal" calcium release operated by membrane voltage in frog skeletal muscle. J. Physiol. 501: 289-303,

Ferreira, G., Yi, J., Ríos, E., and Shirokov, R. Ion-dependent inactivation of barium current through L-type calcium channels. J. Gen. Physiol. 109:449-462, 1997.

Shirokova N and Ríos, E. Small event Ca2+ release: a probable precursor of Ca2+ sparks in frog skeletal muscle. J. Physiol. 502: 3-11, 1997.

Stern, M.D., G. Pizarro and E. Ríos. A local control model of excitation-contraction coupling in skeletal muscle. J. Gen. Physiol. 110: 415-440, 1997.

Shirokov, R., Ferreira, G., Yi, J. and Ríos,E. Inactivation of gating currents of L-type calcium channels. Specific role of the 2 subunit. J. Gen. Physiol. 111:807-823, 1998.

Zhou, J., Cribbs, L., Yi, J., Shirokov, R., Perez-Reyes, E. and Ríos, E. Molecular cloning and functional expression of a skeletal muscle dihydropyridine receptor form Rana catesbeiana. J. Biol. Chem. 273:25503-25509, 1998.

Shirokova, N., J. García and E. Ríos. Local calcium release in mammalian skeletal muscle. J. Physiol. 812:377-384, 1998.

Cheng, H., L.S. Song, N. Shirokova, A Gonzalez, e.G. Lakatta , E. Ríos and M.D. Stern. Amplitude distribution of calcium sparks in confocal images. Theory and studies with an automatic detection method. Biophys. J. 76: 606-617, 1999.

Mejía-Alvarez, R., C. Kettlun, E. Ríos, M.D. Stern and M. Fill. Unitary Ca2+ Current through Cardiac Ryanodine Receptor Channels under Quasi-Physiological Ionic Conditions. J. Gen. Physiol. 113: 177-186, 1999.

Stern, M.D., L. Song, H. Cheng, J. Sham, HT Yang, K.R. Boehler and E. Ríos. Local control models of cardiac excitation-contraction coupling: a possible role for allosteric interactions between ryanodine receptors. J. Gen. Physiol. In press, 1999.

Stern, M.D., L. Song, H. Cheng, J. Sham, HT Yang, K.R. Boehler and E. Ríos. Local control models of cardiac excitation-contraction coupling: a possible role for allosteric interactions between ryanodine receptors. J. Gen. Physiol. 113: 469-490, 1999.

Shirokova, N., A. Gonzalez, W.G. Kirsch, E. Ríos, G. Pizarro, M.D. Stern and H. Cheng. Calcium Sparks: release packets of uncertain origin and fundamental role. J. Gen. Physiol. 113: 377-384, 1999.

Rios, E., M.D. Stern, A. Gonzalez, G. Pizarro, and N. Shirokova. Calcium release flux underlying Ca2+ sparks of frog skeletal muscle. J. Gen. Physiol. 114:31-48.1999.

Shirokova, N., R. Shirokov, D. Rossi, A. Gonzalez, W.G. Kirsch, J. Garcia, V. Sorrentino and E. Ríos. Spatially segregated control of Ca2+ release in developing skeletal muscle. J. Physiol. 521: 483-495. 1999.

A. Gonzalez, W.G. Kirsch, N. Shirokova, G. Pizarro, M.D. Stern and E. Rios. The spark and its ember: separately gated components of Ca release in skeletal muscle. J. Gen. Physiol. 115:139-158. 2000.

A. González, W.G. Kirsch, N. Shirokova, G. Pizarro‡, G. Brum, I.N. Pessah, M.D. Stern, H. Cheng and E. Ríos. Involvement of multiple intracellular release channels in calcium sparks of skeletal muscle. PNAS USA, 97:4380-4385. 2000.

G. Brum, A. Gonzalez, J. Rengifo, N. Shirokova and E. Ríos Fast imaging in three dimensions resolves extensive sources of Ca sparks in frog skeletal muscle. J. Physiol. 2000.

E. Ríos, N. Shirokova, W.G. Kirsch, G. Pizarro, M.D. Stern, H.Cheng, and A. González. .2001.A preferred amplitude of Calcium Sparks in skeletal muscle. Biophys. J. 80: 169-183, 

SQ Wang, LS Song, L Xu, G Meissner, EG Lakatta, MD Stern, E Ríos, H Cheng.  2002.  Thermodynamically irreversible gating of ryanodine receptors in situ, revealed by stereotyped release in Ca2+ sparks.  Biophys. J.  83: 242-251.

J. Rengifo, R. Rosales, A. González, H. Cheng, M.D. Stern, and E. Ríos .  2002.  Ca release as irreversible Markov Process.  Biophys J. 83:2511-2521.

G Ferreira, E. Ríos and N. Reyes.  2003.  Two components of voltage-dependent inactivation in Cav 1.2 channels revealed by its gating currents.  Biophys. J. 84:3662-78.

J. Zhou, G. Brum, A. González, B.S. Launikonis, M.D. Stern, E. Ríos.  2003. Ca2+ sparks and embers of mammalian muscle.  Properties of the sources.  J. Gen. Physiol.  122:95-114.

C Kettlun, A González, E. Ríos and M Fill.  2003.  Unitary Ca current through mammalian cardiac and amphibian skeletal muscle ryanodine receptor channels under near-physiological ionic conditions.  J. Gen. Physiol. 122: 407-417.

SQ Wang, MD Stern, E Ríos, H Cheng.  2004. The Quantal Nature of Ca2+ Sparks and in situ operation of the ryanodine receptor array in cardiac cells. P.N.A.S. USA 101: 3979-3984. 

Csernoch L, Zhou J, Stern MD, Brum G, Rios E.  2004.  The elementary events of Ca2+ release elicited by membrane depolarization in mammalian muscle.  J Physiol. 557:43-58.

Wang, S., Rios, E., Stern, M.D., and Cheng, H.  The Quantal Nature of Ca2+ Sparks and in Situ Operation of the Ryanodine Receptor Array.  PNAS USA.  101:3979-3984.  2004.

Csernoch L, Zhou, J, Stern, M.D., Brum, G., Rios, E.  The elementary events of Ca2+ release elicited by membrane depolarization in mammalian muscle.  J. Physiol. 557:43-58, 2004.

Pizarro, G. and E. Ríos.  How source content determines intracellular Ca2+ release kinetics. Simultaneous measurement of [Ca2+] transients and [H+] displacement in skeletal muscle.  J. Gen. Physiol.  124:239-58, 2004.

Zhou, J., Launikonis, B.S., Rios, E., Brum, G.  Regulation of Ca2+ Sparks by Ca2+ and Mg2+ in Mammalian and Amphibian Muscle. An RyR Isoform-specific Role in Excitation-Contraction Coupling? J. Gen. Physiol. 124:409-28, 2004.

Launikonis, B.S., et al. Confocal imaging of [Ca2+] in cellular organelles by SEER.  2005. Shifted Excitation and Emission Ratioing of fluorescence. J. Physiol. 567:523-543. 2005.      

Zhou, J., et al.    Concerted vs. Sequential. Two Activation Patterns of Vast Arrays of Intracellular Ca2+ Channels in Muscle.  J. Gen. Physiol. 126:301-9, 2005.

Zhou, J., et al.  A probable role of dihydropyridine receptors in repression of Ca2+ sparks, demonstrated in cultured mammalian muscle.  Am. J. Physiol. (Cell). 290:C539-C553. 2006.

Launikonis, B.S. et al.  Depletion “skraps” and dynamic buffering inside the cellular calcium store.  Proc.Nat.Acad.Sci. USA.  103:2982-2987. 2006.

Launikonis, B.S. et al.  The Changes in Ca2+ Sparks Associated with Measured Modifications of Intra-store Ca2+ Concentration in Skeletal Muscle. J Gen Physiol. 2006. 128:45-54.

Curran, J.  et al.  b-Adrenergic Enhancement of Sarcoplasmic Reticulum Ca Leak in Cardiac Myocytes Is Mediated by Ca-Calmodulin Dependent Protein Kinase.  2007 Circ. Res. 2007 100:391-8.

Pouvreau S, et al.  Ca2+ sparks operated by membrane depolarization require isoform 3 ryanodine receptor channels in skeletal muscle. PNAS U S A. 2007 104:5235-40. Erratum in: PNAS U S A. 2007.

Launikonis BS, Rios E.  Store-operated Ca2+ entry during intracellular Ca2+ release in mammalian skeletal muscle. J Physiol. 2007; 583:81-97.

Ríos E, Zhou J, Brum G, Launikonis BS, Stern MD.Calcium-dependent inactivation terminates calcium release in skeletal muscle of amphibians.J Gen Physiol. 2008 Apr;131(4):335-48.

Musset, D. Morgan, V.V. Cherny, D.W. MacGlashan, L.L. Thomas, E. Ríos, T.E. DeCoursey.  Voltage-Gated Proton Channels in Human Basophil Responses.  P.N.A.S. U S A., 105: 11020-5. 2008.

Royer L, Pouvreau S, Ríos E..  Evolution and modulation of intracellular calcium release during long-lasting, depleting depolarization in mouse muscle.  J Physiol. 586:4609-29. 2008

Deconstructing calsequestrin. Complex buffering in the calcium store of skeletal muscle. Royer L, Ríos E. J Physiol. 587:3101-11. 2009.

Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis.  Morgan D, Capasso M, Musset B, Cherny VV, Ríos E, Dyer MJ, DeCoursey TE. Proc Natl Acad Sci U S A. 106:18022-7. 2009

Calsequestrin, triadin and more: the molecules that modulate calcium release in cardiac and skeletal muscle. Ríos E, Györke S. J Physiol. 587:3069-70. 2009

Indo-1 derivatives for local calcium sensing.  M. Bannwarth, IR Correa, M Sztretye, S Pouvreau, C Fellay, A Aebischer, L Royer, E Rios, K Johnsson. ACS Chemical Biology 4: 3, 2009. Cover Article.

RyR1 Expression and the Cell Boundary Theorem Ríos E. J Biol Chem. 285: le13.  2010.     

Paradoxical buffering of calcium by calsequestrin demonstrated for the calcium store of skeletal muscle.  Royer L, Sztretye M, Manno C, Pouvreau S, Zhou J, Knollmann BC, Protasi F, Allen PD, Ríos E.  J Gen Physiol. 136:325-38.2010 

The cell boundary theorem: a simple law of the control of cytosolic calcium concentration. Ríos E. J Physiol Sci. 60:81-84. 2010

Ca sparks do not explain all ryanodine receptor-mediated SR Ca leak in mouse ventricular myocytes.  Santiago DJ, JW Curran, DM Bers, WJ Lederer, MD Stern, E Ríos, TR Shannon. Biophys J. 98:2111-2120. 2010.

Hyperactive intracellular calcium signaling associated with localized mitochondrial defects in skeletal muscle of an animal model of amyotrophic lateral sclerosis.  Zhou J, Yi J, Fu R, Liu E, Siddique T, Ríos E, Deng HX.    J. Biol. Chem. 285:705-712. 2010.

Mitochondrial calcium uptake regulates rapid calcium transients in skeletal muscle during excitation-contraction (E-C) coupling. Yi, J, C Ma, Y Li, N Weisleder, E Ríos, J Ma and J Zhou.   J. Biol. Chem. 286:32436-32443. 2011.

D4cpv-calsequestrin: a sensitive ratiometric biosensor accurately targeted to the calcium store of skeletal muscle.  Sztretye, M, J Yi, L Figueroa, J Zhou, L Royer and E Ríos .  J. Gen. Physiol. 138:211-229.  2011.

Measurement of RyR permeability reveals a role of calsequestrin in termination of SR Ca2+ release in skeletal muscle.  Sztretye, M, J Yi, L Figueroa, J Zhou, L Royer, P Allen, G Brum and E Ríos .  J. Gen. Physiol. 138:231-247.  2011.

Mitochondrial calcium uptake regulates rapid calcium transients in skeletal muscle during excitation-contraction (E-C) coupling. Yi J, Ma C, Li Y, Weisleder N, Ríos E, Ma J, Zhou J.  J Biol Chem.  Sep 16;286(37):32436-43. 2011.

Properties of Ca2+ sparks revealed by four-dimensional confocal imaging of cardiac muscle. Shkryl, V, Blatter, L and E Rios.  J. Gen. Physiol.  139:189-207  2012.  Cover Article.

Synthetic localized calcium transients directly probe signaling mechanisms in skeletal muscle.  Figueroa, L, VM Shkryl, J Zhou, C Manno, A Momotake, G Brum , LA Blatter, GCR Ellis-Davies and E Ríos.    J. Physiol.  590: 1389-1411. 2012.  Cover Article.

see also  Waveless mammalian muscle.  Jacquemond V 2012.  J. Physiol 590:1783.

Altered Ca2+ concentration, permeability and buffering in the myofibre Ca2+ store of a mouse model of malignant hyperthermia. Manno C, Figueroa L, Royer L, Pouvreau S, Lee CS, Volpe P, Nori A, Zhou J, Meissner G, Hamilton SL, Ríos E. J Physiol. 591(Pt 18):4439-57; 2013 .

Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. Manno C, Figueroa L, Fitts R, Ríos E.
J Gen Physiol.  141(3):371-87; 2013.

Using two dyes with the same fluorophore to monitor cellular calcium concentration in an extended range.
Figueroa L, Shkryl VM, Blatter LA, Ríos E. PLoS One. ;8(2):e55778; 2013.

Dynamic measurement of the calcium buffering properties of the sarcoplasmic reticulum in mouse skeletal muscle.
Manno C, Sztretye M, Figueroa L, Allen PD, Ríos E.
 J Physiol.  591(Pt 2):423-42.   2013.

Life and death of a cardiac calcium spark. Stern MD, Ríos E, Maltsev VA. J Gen Physiol.  142(3):257-74. 2013.

Isoproterenol increases the fraction of spark-dependent RyR-mediated leak in ventricular myocytes. Santiago DJ, Ríos E, Shannon TR. Biophys J.  104(5):976-85. 2013

El¨"cuplón", unidad funcional del acoplamiento contráctil.  E Rios.  An Facultad Med (Univ. Repúb. Urug.). 2014; 1(1):4-28.  2014.

A better method to measure total calcium in biological samples yields immediate payoffs. Manno C, Ríos E. J Gen Physiol.  145(3):167-71. 2015.

The couplonopathies: A comparative approach to a class of diseases of skeletal and cardiac muscle. Ríos E, Figueroa L, Manno C, Kraeva N, Riazi S. 2015. Viewpoint. J Gen Physiol. 2015 Jun;145(6):459-74. doi:0.1085/ jgp.201411321. PMID: 26009541.

 


Multimedia:


Rios E. The Cell Boundary Theorem. Henry Stewart Lectures on Calcium Signaling. http://hstalks.com/main/view_talk.php?t=2210&r=13&c=252

Rios E. Did Alan Hodgkin dream of calcium sparks? http://jgp-soundsphys.rupress.org/2015/05/25/eduardo-rios/ May 25, 2015 - A conversation with Eduardo Ríos on post-Hodgkin thought, the voltage sensor in EC coupling, calcium sparks, and the university system in Uruguay. Podcast by Elizabeth Adler, Executive Editor, Journal of General Physiology.

 

Invited papers and book chapters:

Ríos, E. Excitation contraction coupling in skeletal muscle. Problems and advances. Boletín de la Sociedad de Biofísicos Latino Americanos. 2: 4-5, 1988 (in Spanish).
Pizarro, G., Brum, G., Fill, M., Fitts, R., Rodríguez, M., Uribe, I. and Ríos, E. The voltage sensor of skeletal muscle excitation-contraction coupling. A comparison with Ca channels. "The calcium channel: Structure, Function and Implications". Edited by M. Morad, W. Nayler, S. Kazda, M. Schramm. pp 138-158. Springer-Verlag, Heidelberg, 1988.

Ríos, E. and Pizarro, G. The voltage sensors and calcium channels of excitation-contraction coupling. News in Physiological Sciences, 3: 223-227, 1988.

Ríos, E., Pizarro, G., Uribe, I. and Fitts, R. A third role for calcium in excitation-contraction coupling. In: "Transduction in Biological Systems". Edited by C. Hidalgo, J. Bacigalupo, E. Jaimovich an J. Vergara, pp 385-400. Plenum Press, New York, 1990.

Ríos, E. and G. Pizarro. The voltage sensor of E-C coupling in skeletal muscle. Physiological Reviews, 71: 849-908, 1991.

Ríos, E., J. Ma and A. González. The mechanical model of excitation-contraction coupling in skeletal muscle. Journal of Muscle Research and Cell Motility, 12: 127-135, 1991.

Hosey, M.M., Brawley, R.M., Chang, C.F., Gutiérrez, L.M., Mundiña-Weilenmann, C., Ma, J. and Ríos, E. Phosphorylation and regulation of calcium channels by multiple protein kinases. In: "NATO Advance Research Workshop on Intracellular Regulation of Ion Channels". Edited by Martin Morad. Springer-Verlag, 1991.

Ríos, E., Pizarro, G. and E. Stéfani. Charge movements and the nature of signal transduction in skeletal muscle excitation-contraction coupling. Annual Review of Physiology, 54: 109-133, 1992.

Csernoch, L., Pizarro, G., Garcia, J., Szucs, G., Stefani, E. and Ríos, E. Effects of sarcoplasmic calcium release on intramembrane charge movement in skeletal muscle. In: "Excitation-Contraction Coupling in Skeletal, Cardiac, and Smooth Muscle". Edited by G.B. Frank et al. pp 137-148. Plenum Press, New York, 1992.

Ríos, E. Reining in Calcium Release. Biophys. J. 67: 7-9, 1994.

Ríos, E. Homeostasis of Ca2+ in skeletal muscle. In "Nerve-Muscle Function: Bioelectro- Chemistry, Mechanism, Energetics and Control". Edited by G. Milazzo. NATO-ASI, Roma, 1995.

Ríos, E., González, A., Karhanek, M., Ma, J., Shirokov, R., Pizarro, G., Csernoch, L., Fitts, R., Uribe, I., Hosey, M.M. Excitation-contraction coupling in skeletal and cardiac muscle. In: "Nerve-Muscle Function: Bioelectro- Chemistry, Mechanism, Energetics and Control". Edited by G. Milazzo. NATO-ASI, Roma. 1995.

Ríos, E. Excitation contraction coupling in skeletal muscle. In: "Biofísica". Edited by Ramón Latorre, Universidad de Sevilla Press. 1996.

Ríos, E. Optical techniques for detection of intracellular calcium. In: "Biofísica". Edited by Ramón Latorre, Universidad de Sevilla Press. 1996.

Ríos, E. and Stern, M. Calcium in Close Quarters: Microdomain feedback in excitation-contraction coupling and other cell biological phenomena. Ann. Rev. Biophys. Biomolec. Struct.,26:47-82 1997.

Calcium Sparks: release packets of uncertain origin and fundamental role. Shirokova, N., A. Gonzalez, W.G. Kirsch, E. Ríos, G. Pizarro, M.D. Stern and H. Cheng. J. Gen. Physiol. 113:377-384, 1999.

Ríos, E. and G. Brum. Ca2+ release flux underlying Ca2+ transients and Ca2+ sparks in skeletal muscle.  Frontiers in Bioscience 7, d1195-1211,  2002

Ríos, E.  and A. González.  Excitation-Contraction Coupling in Skeletal Muscle.  In: “Molecular Control Mechanisms in Muscle Contraction”.  Edited by Richard Moss and John Solaro.  Kluver Academic Publishers.  Dordrecht, 2002.  PP 1-48.

 

Rios E, Zhou J. Control of dual isoforms of Ca2+ release channels in muscle. Biol Res. 37:583-91. 2004

 

Rios E, Launikonis BS, Royer L, Brum G, Zhou J. The elusive role of store depletion in the control of intracellular calcium release.
J Muscle Res Cell Motil. 27:337-50. 2006.

 

Rios E.  Calcium signalling in muscle: a milestone for modulation studies. J Physiol. 2006; 572:1-2. 

 

 

Book

Ríos, E. Transport and Excitability, Functions of Cell Membranes. (In Spanish). Department of Publications, Universidad de la República, Montevideo, Uruguay, 1981. This is an introductory textbook on membrane physiology (still!) used in several schools of the Universidad de la República.


Other


I write a column titled "Chronicles from the Far North", of irregular frequency, in "Batoví", an Uruguayan weekly. In it, I share with my former fellow citizens the experience of migration, and societal and cultural contrast.



Selected Lectures at International Symposia (updated 2006):


Excitation Contraction Coupling. Instituto Mexicano de Cardiología, Mexico, 1978.


Antipyrylazo III measures and modifies Ca transients in skeletal muscle fibers. Guanajuato, Mexico, 1981. Satellite Symposium of the 7th International Biophysics Congress.


Time course of Ca release from the sarcoplasmic reticulum. Gordon Conference on Muscle; Tilton, New Hampshire, 1983.


Kinetics of Ca release: an experimental measurement of the transmission time at the triadic gap. Gordon Conference on Muscle; Tilton, New Hampshire, 1985.


Effects of calcium on calcium release and intramembrane charge movements in skeletal muscle. Gordon Conference on Muscle; Tilton, New Hampshire, 1985.


Calcium, calcium blockers and excitation-contraction coupling. Symposium on Muscle. Ruhr University, Department of Physiology, Bochum, West Germany, 1986.


Modulation of intramembrane charge movement and Ca release from the Sarcoplasmic Reticulum. Symposium on Muscle. FASEB, 71st Annual Meeting, Washington, DC, 1987.


Voltage sensitivity of ion channels. Latin American Federation of Physiological Sciences. Facultad de Ciencias, Montevideo, Uruguay, May 7, 1988.

A third role of calcium in skeletal muscle excitation-contraction coupling. Workshop: Signal Transduction in Biological Systems". Valparaiso, Chile, May 30, 1988.


Gordon Conference: Skeletal Muscle EC coupling. Plymouth, N.H., 1988. Leader of the Session: Intramembrane Charge Movement, Ca and Membrane Proteins.


Intramembrane Charge Movements in Rat Cardiac Myocytes. Annual Meeting of the International Society for Heart Research. Williamsburg, Virginia, June 27, 1988.

The voltage sensor of excitation-contraction coupling. A comparison with Ca channels. In "Molecular Aspects of Excitation-Contraction Coupling." Workshop of the Biophysical Society. Cincinnati, February 13, 1989.

From TT to SR and Back. Transmission in skeletal muscle excitation-contraction coupling. 1st Ibero American Congress of Biophysics. Sevilla, Spain, September 1989.

Positive feedback in excitation-contraction coupling. Department of Physiology, University Medical School. Debrecen, Hungary. September 29, 1989.

Positive feedback in excitation-contraction coupling. Centre of Physiological Sciences. Slovak Academy of Sciences. Bratislava, Chekoslovakia. October 2, 1989.

Excitation-contraction coupling in skeletal muscle. Marquette Life Sciences Symposium. Marquette University, Milwaukee. March 1990.

Intramembrane charge movements in Excitation-Contraction coupling. Session leader, Gordon Conference on Muscle, Tilton, N.H. 1991.

The dihydropyridine receptors of skeletal and cardiac muscle, a comparison of functional aspects. American Physiological Society Symposium "From channels to cross bridges." Mt. Desert Island, Maine, 1991.

Calcium homeostasis in skeletal muscle. International School of Biophysics. Erice, Italy, 1991.

Excitation-contraction coupling in skeletal and cardiac muscle. International School of Biophysics. Erice, Italy, 1991.

Cellular mechanisms of excitation-contraction coupling. Symposium on Muscle Fatigue, The American Physiological Society, Augusta, GA, 1992.

Excitation-contraction coupling in skeletal muscle. Elements of a molecular picture. Biomembrane subgroup, Annual Meeting of the Biophysical Society, Houston, TX, 1997.

An allosteric model of excitation-contraction coupling. Symposium on Muscle. Meeting of the International Union of Physiological Sciences. Glasgow, 1993.

An allosteric model of excitation-contraction coupling. Symposium on Signal Transduction. Meeting of the International Union of Pure and Applied Biophysics. Budapest, 1993

Current concepts on Excitation-contraction coupling in skeletal muscle. Annual Meeting of the Biophysical Society, Contractility Subgroup. New Orleans, LA, 1994.

Signal transduction in skeletal and cardiac muscle. Latin American Association of Physiological Sciences. Montevideo, Uruguay, 1994.

Measurement of intracellular calcium. International course on signal transduction. Debrecen, Hungary, 1994.

Excitation-Contraction Coupling. Invited Lecture at the European Muscle Club. Ruhr University, Bochum, Germany, 1994.

Imaging Elementary Events of Ca2+ Release. Annual Symposium of the Society of General Physiologists. Woods Hole, MA, 1995.

Microdomain control of Ca release. FASEB symposium on "Local Ca Signaling". Washington, D.C., 1996.

Ca2+ signaling in muscle. Keynote address to the TriBeta Research Meeting, Univ. Wisconsin at Stevens Point, 1997.

Ca2+ sparks and the control of Ca2+ release in skeletal muscle. 1st K-JIST International Symposium on Life Science:"Excitation-Contraction Coupling". Seoul, South Korea, 1998.

Sparks, quarks and E-C coupling. The University of Tokyo. 1998.

True distribution of spark sizes in skeletal muscle. Annual Symposium of the Society of General Physiologists. Woods Hole, Massachusetts, 1998.

Ca sparks and couplons: functional emergents of Ca release in muscle. The Latin American Federation of Physiological Societies. Cancún, Mexico, 2000.

Ca sparks and couplons: functional emergents of Ca release in muscle. The Ibero American congress of biophysics. Alicante, Spain, 2000.

The significance of Ca sparks in E-C Coupling. Symposium of the Biophysical Society, Boston, 2001.

Sparks, so what? Keynote Address, the Midwest Physiological Society. Madison, Wisconsin, 2001.

From molecular events to cellular function in excitation-contraction coupling. The European Muscle Society, Pavia, Italy, 2001.

Ca2+ sparks of mammalian muscle. Initial studies. The International Union of Pure and Applied Biophysics. Satellite Symposium. Montevideo, 2002.

Control of dual isoforms of Ca2+ release channels in muscle.  International Symposium on Intracellular Ca Channels.  Marbella Resort, Chile, 2003.

Ca control inside the SR.  Plenary Lecture.  The Ibero-American congress of Biophysics.  Rio de Janeiro, Brasil, 2003.

Concerted and sequential activation of Ca release channels.  The Gordon Conference on Muscle. New Hampshire, 2003.

Ca control inside the Sarcoplasmic Reticulum.  Symposium on Calcium Signaling.  Biophysical Society meeting.  Long Beach, 2005.

The elusive role of store depletion in the control of intracellular calcium release.  Symposium on Excitation-Contraction Coupling, satellite to the European Muscle Conference, Heidelberg, Germany, 2006.

 

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