CURRICULUM VITAE

THOMAS R. SHANNON

11/28/06

CURRENT ADDRESSES

 

Department of Molecular Biophysics and Physiology

Rush University

1750 West Harrison Street

Chicago, Illinois  60612                                (312)942-2213

Email: tshannon@rush.edu

 

899 S. Plymouth Ct.

Unit 1708

Chicago, Illinois 60605                                 (630)886-2601

 

 

PERSONAL INFORMATION

Birth Date and Place: January 19, 1964, Cincinnati, Ohio

Citizenship: United States

Marital Status: Single

 

 

DEGREES EARNED

D.V.M. from University of Missouri, Columbia, Missouri, May 1989

Ph.D. from University of Missouri, Columbia, Missouri, December 1993

 

POSITIONS HELD

Assistant Professor, Department of Molecular Biophysics and Physiology, Rush University, Chicago, Illinois, 2002-present

 

Research Assistant Professor, Department of Physiology, Loyola University-Chicago, Maywood, Illinois, 2000-2002.

 

Research Associate, Department of Physiology, Loyola University-Chicago, Maywood, Illinois, 1993-2000.

 

Postdoctoral Fellow, Department of Veterinary Biomedical Sciences, University of Missouri, Columbia, Missouri, 1989-1993.

 

Technical Assistant, Research Animal Diagnostic and Investigative Laboratory, University of Missouri, Columbia, Missouri, 1988-1989.

 

Assistant Librarian, University of Missouri Veterinary Teaching Hospital, Columbia, Missouri, 1985-1989.

 

Technical Assistant, University of Missouri Veterinary Teaching Hospital, Columbia, Missouri, 1984-1987.

 

HONORS

Ralph S. Zitnik, M.D. Clinical Research Investigatorship, American Heart Association, Metropolitan Chicago Affiliate, 1998 (awarded to highest scoring application)

University of Missouri College of Veterinary Medicine Annual Poster Competition (2nd place), 1991

Licensed Veterinarian- State of Missouri, 1989-present

Dean's List, 1982-1986

Recipient of Westlake Scholarship, 1982-1985

 

 

CURRENT RESEARCH INTERESTS

Physiology and pharmacology of cellular ion movement and storage

Physiology and pharmacology of transport

Excitation-contraction coupling

Ion-motive ATPases

Sodium-calcium exchange

 

GRANT HISTORY

            National Institutes of Health, R01 award,  $200,000/yr, 2003-present

American Heart Association-Midwest Affiliate, Scientist Development Grant, $80,000/year, 2000-2003

American Heart Association of Metropolitan Chicago, Senior Research Fellowship, 1998-2000

National Institutes of Health Individual National Research Service Award, 1995-1998

American Heart Association of Metropolitan Chicago, Junior Research Fellowship, 1995 (Funded but returned in favor of NRSA above)

American Heart Association-Missouri Affiliate Research Fellowship, 1993-1994

Pharmaceutical Manufacturers Association Research Fellowship, 1991-1993 (Last year funded but declined in favor of AHA fellowship above)

 

TEACHING

Medical Physiology (Physiology 451), Rush University School of Medicine, Interim Course Director, 51 contact hours, 2005

Medical Physiology (Physiology 451), Rush University School of Medicine, 28 contact hours, 2004

Medical Physiology (Physiology 451), Rush University School of Medicine, 28 contact hours, 2003

Medical Physiology (Physiology 451), Rush University School of Medicine, 12 contact hours, 2002

Molecular and Cellular Biology, Loyola University-Chicago School of Medicine, 2 weeks, 1999

Molecular and Cellular Biology, Loyola University-Chicago School of Medicine, 2 weeks, 1998

Veterinary Cellular Biology and Biochemistry, University of Missouri College of Veterinary Medicine, 4 weeks, 1991, 1992

 

COMMITTEES

Graduate Professional Council, 1990-1993

Graduate Student Association, 1990-1992 (Treasurer, 1991-1992)

Research and Creative Activities Forum Steering Committee, 1990-1992

 

PROFESSIONAL SOCIETIES

Biophysical Society, 1992-present

American Veterinary Medical Association, 1989-1991

Missouri Veterinary Medical Association, 1989-1991

Student Chapter of the American Veterinary Medical Association, 1985-1989

 

MEETINGS ATTENDED

            American Heart Association Scientific Sessions, Chicago, IL 2006 (session chair)

            Annual Meeting of the Biophysical Society, Baltimore, MD, 2006

            Gordon Conference on Cardiac Regulatory Mechanisms, New London, NH, 2006     Gordon Conference on Excitation-Contraction Coupling, New London, NH, 2006         American Heart Society Scientific Sessions, Dallas, TX, 2005

            Annual Meeting of the Biophysical Society, Long Beach, CA, 2005

            Annual Meeting of the Biophysical Society, Baltimore, MD, 2004

Gordon Conference on Cardiac Regulatory Mechanisms, New London, NH, 2004 (invited speaker)

Annual Meeting of the Biophysical Society, Baltimore, MD, 2004

            American Heart Society Scientific Sessions, Orlando, FL, 2004

            Annual Meeting of the Biophysical Society, San Antonio, TX, 2003

American Heart Society Scientific Sessions, Chicago, IL, 2002

Gordon Conference on Cardiac Regulatory Mechanisms, New London, CT, 2002 (invited speaker)

Annual Meeting of the Biophysical Society, San Francisco, CA, 2002

American Heart Society Scientific Sessions, Anaheim, CA, 2001

Annual Meeting of the Biophysical Society, Boston, MA, 2001

Gordon Conference on Cardiac Regulatory Mechanisms, New London, NH, 2000

Annual Meeting of the Biophysical Society, Baltimore, MD, 2000

Meeting of the Midwest Physiological Society, Madison, WS, 1999

Annual Meeting of the Biophysical Society, Baltimore, MD, 1999

Annual Meeting of the Biophysical Society, Kansas City, MO, 1998

Gordon Conference on Cardiac Regulatory Mechanisms, New London, NH, 1998

New York Academy of Sciences, Cardiac Sarcoplasmic Reticulum Function and Regulation of Contractility, Washington, D.C., 1997

Annual Meeting of the Biophysical Society, New Orleans, LA, 1997

Annual Meeting of the Biophysical Society, Baltimore, MD, 1996

Annual Meeting of the Biophysical Society, San Francisco, CA, 1995

Annual Meeting of the Biophysical Society,  New Orleans, LA, 1994

Annual Meeting of the American Societies for Experimental Biology, New Orleans, LA, 1993

Annual Meeting of the Biophysical Society, Washington, D.C., 1993

Annual Meeting of the Biophysical Society, Houston, TX, 1992

Annual Meeting of the Biophysical Society, Baltimore, MD, 1990

 

 

PUBLICATIONS

PAPERS

Shannon, T.R., Milanick, M.A., Hale, C.C. (1994) Interaction of cardiac Na-Ca exchanger and exchange inhibitory peptide with membrane phospholipids.  Am. J. Physiol.  266:C1350-C1356.

 

Shannon, T.R., Hale, C.C. (1994) Identification of a 65 kDa endothelin receptor in bovine cardiac sarcolemmal vesicles. Eur. J. Pharmacol.  267:233-238.

 

McCall, E., Li, L., Satoh, H., Shannon, T.R., Blatter, L.A., Bers, D.M. (1996) Effects of FK-506 on contraction and Ca transients in rat cardiac myocytes.  Circ. Res. 79:1110-1121.

 

Qi, M., Shannon, T.R., Euler, D.E., Bers, D.M., Samarel, A.M. (1997) Downregulation of sarcoplasmic reticulum Ca2+-ATPase during progression of left ventricular hypertrophy.  Am J. Physiol. 272:H2416-H2424.

 

Shannon, T.R., Bers, D.M.  (1997) Assessment of intra-SR free [Ca] and buffering in rat heart.  Biophys. J. 73:1524-1531.

 

McCall, E., Ginsburg, K.S., Bassani, R.A., Shannon, T.R., Qi, M., Samarel, A.M., Bers, D.M. (1997)  Ca flux, contractility and excitation-contraction coupling in hypertrophic rat ventricular myocytes.  Am. J. Physiol. 274:H1348‑1360.

 

Shannon, T.R., Ginsburg, K.S., Bers, D.M. (1998)  Reverse mode of the SR Ca pump limits SR Ca uptake in permeabilized and voltage clamped myocytes.  Ann. N.Y. Acad. Sci. 853:350-352.

 

Cadre B.M., Qi, M., Eble, D.M., Shannon, T.R., Bers, D.M., Samarel, A.M. (1998)  Cyclic stretch down‑regulates calcium transporter gene expression in neonatal rat ventricular myocytes.  J. Mol. Cell. Cardiol. 31:2247‑2259.

 

Bassani, R.A, Shannon, T.R., Bers, D.M. (1998)  Passive Ca2+ binding in ventricular myocardium of neonatal and adult rats.  Cell Calcium 23:433‑42.

 

Shannon, T.R., Ginsburg, K.S., Bers, D.M.  (2000) Reverse mode of the sarcoplasmic reticulum calcium pump and load-dependent cytosolic Ca decline.  Biophysical J. 78:322-333.

 

Shannon, T.R., Ginsburg, K.S., Bers, D.M.  (2000) Potentiation of fractional SR Ca release by total and free intra-SR Ca concentration.  Biophysical J.  78:334-343.                                                        

 

Frank, K., Tilgmann, C., Shannon, T.R., Bers, D.M., Kranias, E.G.  (2000) Regulatory role of phospholamban in the efficiency of cardiac sarcoplasmic reticulum Ca2+-transport.  Biochemistry  39:14176-14182.

 

Shannon, T.R., Kranias, E.G., Bers, D.M.  (2001)  Phospholamban decreases the energetic efficiency of the SR Ca pump.  J. Biol. Chem.  276:7195-7201.

 

Weber, C.R., Ginsburg, K.S., Philipson, K.D., Shannon, T.R., Bers, D.M.  (2001) Allosteric regulation of Na/Ca exchange current by cytosolic Ca in cardiac myocytes.  J. Gen. Physiol. 117:119-132.

 

Shannon, T.R., Ginsburg, K. S.  Bers, D.M. (2002)  Quatitative Assessment of the SR Ca Leak-Load Relationship.  Circ. Res. 91:594-600.

 

Shannon, T.R., Guo, T., Bers, D.M. (2003) Ca2+ Scraps: Local Depletions of Free [Ca2+] in Cardiac Sarcoplasmic Reticulum during Contractions Leave Substantial Ca2+ Reserve.  Circ. Res. 93:40-5.

 

Shannon, T.R., Pogwizd, S. M., Bers, D.M. (2003) Elevated Sarcoplasmic Reticulum Ca Leak in Intact Ventricular Myocytes from Rabbits in Heart Failure. Circ. Res. 93:592-594.

 

Shannon, T.R., Bers, D. M. (2004)  Integrated Ca2+ Management in Cardiac Myocytes.  Ann. N.Y. Acad. of Sci. 1015:28-38

 

Shannon, T.R., Wang, F., Puglisi, J., Weber, C.W., Bers, D.M. (2004)  A Mathematical Treatment of Integrated Ca Dynamics Within the Ventricular Myocyte.  Biophys. J. 87:1-21.

 

Launikonis, B.S., Zhou, J., Royer, L., Shannon, T.R., Brum, G., Rios, E.  (2005)  Confocal imaging of [Ca2+] in cellular organelles by SEER, shifted excitation and emission ratioing of fluorescence.  J. Physiol. 567:523-43.

 

Shannon, T.R., Wang, F., Bers, D.M.  (2005)  Regulation of Cardiac Sarcoplasmic Reticulum Ca Release by Luminal [Ca] and Altered Gating Assessed with a Mathematical Model.  Biophys. J. 89:4096-4110.

 

Ai X, Curran JW, Shannon T.R., Bers DM, Pogwizd SM. (2005) Ca2+/Calmodulin-Dependent Protein Kinase Modulates Cardiac Ryanodine Receptor Phosphorylation and Sarcoplasmic Reticulum Ca2+ Leak in Heart Failure.  Circ. Res. 97:1314-1322.

 

Launikonis, B.S., Zhou, J., Royer, L., Shannon, T.R., Brum, G., Ríos, E. (2006)  Depletion “skraps” and dynamic buffering inside the cellular Ca2+ store.  Proc. Nat. Acad. Sci. 103:2982-2987,

 

Curran, J., Hinton, M.J. Ríos, E., Bers, D.M., Shannon, T.R. (2006) b-Adrenergic Enhancement of Sarcoplasmic Reticulum Ca Leak in Cardiac Myocytes Is Mediated by Ca-Calmodulin Dependent Protein Kinase.  Circ. Res. (in press).

 

 

ABSTRACTS

 

Shannon, T.R., Allert, J.A., LeBourveau, L.M., Adams, H.R., Hale, C.C. (1990) Partial characterization of an endothelin receptor in cardiac sarcolemmal vesicles, Biophysical J. 57:288a.

 

Shannon, T.R., Hale, C.C. (1992) Partial characterization of the endothelin-1 receptor binding site in cardiac sarcolemmal vesicles, Biophysical J. 61:A258.

 

Shannon, T.R., Milanick, M.A., Hale, C.C. (1993) Interaction of cardiac Na-Ca exchange and the exchange inhibitory peptide with membrane phospholipids.  Biophysical J.  64:A400.

 

Shannon, T.R., Milanick, M.A., Hale, C.C. (1993) Exchange inhibitory peptide inhibition of Ca-Ca exchange. FASEB J.  7:A358.

 

Shannon, T.R., Hale, C.C., Milanick, M.A.  (1994)  Potency of exchange inhibitory peptide (XIP) inhibition of the Na-Ca exchanger in alternate transport modes.  Biophys. J. 66:A332.

 

Shannon, T.R., Bers, D.M.  (1996)  Intra-SR free Ca in rat cardiac microsomes.  Biophys. J.  70:A55.

 

Hüser, J., Shannon, T.R., Rechenmacher, C.E., Bers, D.M., Blatter, L.A.  (1997)  Confocal microscopic recording of membrane potential in single isolated cardiac mitochondria.  Biophys. J.  72:A160.

 

Shannon, T.R., Ginsburg, K.S., Bers, D.M.  (1997)  SR Ca uptake rate in permeabilized ventricular myocytes is limited by reverse mode of the SR Ca pump.  Biophys. J.  72:A167.

 

Shannon, T.R., Ginsburg, K.S., Bers, D.M. (1998) Rate of [Ca]c decline decreases with increasing SR Ca load in patch clamped cardiac myocytes.  Biophys. J. 74:A56.

 

Shannon, T.R., Ginsburg, K.S., Bers, D.M. (1998)  Fractional SR Ca release is potentiated by increased SR [Ca].   Biophys. J. 74:A248.

 

Shannon, T.R., Chu, G., Kranias, E.G., Bers, D.M. (1999) Rate of SR Ca Leak and the Efficiency of the SR Ca Pump in Nonpentameric Phospholamban Mutant Mice.   Biophys. J. 76:A297.

 

Shannon, T.R., Kranias, E.G., Bers, D.M. (2000) How Does Phospholamban Decrease SR Ca Content?   Biophys. J. 78:A75.

 

Shannon, T.R., Blatter, L.A., Bers, D.M., Niggli, E. (2001)  Ca Release Signals From SR Vesicles Imaged with Confocal Microscopy.  Biophysical J. 80:589a.

 

Shannon, T.R., Bers, D.M. (2001)  A Mathematical Model Describes the SR Load-Dependence of Ca Dynamics in Cardiac Myocytes.  Biophysical J. 80:594a.

 

Shannon, T.R., Ginsburg, K.S., Bers,D.M. (2001)  The Impact of Sarcoplasmic Reticulum (SR) Ca Leak Upon Ca Homeostasis in Intact Isolated Ventricular Myocytes.  Circ. 104:II193.

 

Shannon, T.R., Ginsburg, K. S., Bers, D. M. (2002) Measurement of Load-sensitive Sarcoplasmic Reticulum (SR) Ca Leak in Intact Isolated Ventricular Myocytes. Biophys. J. 82:597a.

 

Shannon, T.R., Guo, T., Bers, D.M.  (2003)  Junctional Free Intra-SR Free [Ca] Declines to ˜50% During Release in Intact Ventricular Myocytes.  Biophys. J.  84:259a.

 

Shannon, T.R., Pogwizd, S.M., Bers, D.M.  (2003)  Sarcoplasmic Reticulum (SR) Ca Leak is Higher in Intact Isolated Ventricular Myocytes from Rabits in Heart Failure.  Circulation 108:IV-48.

Shannon, T.R., Wang, F., Bers, D.M.  (2004)  Evaluation of the Role of Free SR Ca in Cardiac EC Coupling Using a Mathematical Model.  Biophys. J.  86:109a.

 

Curran, J., Rios, E., Bers, D.M. Shannon, T.R.  (2004)  Effects of Adrenergic Stimulation Upon Sarcoplasmic Reticulum Ca Leak in Intact Rabbit Ventricular Myocytes.  Biophys. J.  86:344a.

 

Santiago, D., Rios, E., Shannon, T.R.  (2004)  Permeability Entry of Ca Precipitating Ions Into the Sarcoplasmic Reticulum of Mammalian and Amphibian Twitch Muscle.  Biophys. J.  86:579a.

 

Launikonis, B.S., Zhou, J., Royer, L., Santiago, D.J., Shannon, T.R., Pizarro, G., Brum, G., Ríos, E.  (2005)  Control of Calcium Release by Intra-Store Ca2+.  Biophys. J.  88:13a.

 

Santiago, D.J., Shannon, T.R., Ríos, E., Stern, M.D.  (2005)  Skeletal and Cardiac Ca2+ Sparks “Mano A Mano”. A Comparison of Source Kinetics by Morphometry and Modeling.  Biophys. J.  88:86a.

 

Royer, L., Launikonis, B.S., Zhou, J., Shannon, T.R., Brum, G., Ríos, E.  (2005)  SEER (Shifted Excitation and Emission Ratioing) of Mag-indo Fluorescence. Description, Calibration In Situ and Measure of [Ca2+] and Dye Concentration Inside the SR.  Biophys. J.  88:89a.

 

Wang, F., Despa, S., Ginsburg, K.S., Shannon, T.R., Bers, D.M.  (2005)  Na Diffusion-Dependent Ca Handling in Rabbit and Mouse Ventricular Myocytes.  Biophys. J.  88:137a.

 

Curran, J.W., Bers, D.M., Shannon, T.R.  (2005) Ca-Calmodulin-Dependent Protein Kinase II Phosphoylation of the Ryanodine Receptor Increases SR Ca Leak.  Biophys. J.  88:487a.

 

Guo, T., Shannon, T.R., Pogwizd, S., Bers, D.M.  (2005)  Decreased Intra-SR Free [Ca] in Rabbit Ventricular Myocytes in Heart Failure Model.  Circulation 112:II57.

 

Curran, J., Huke, S., Pogwizd, S., Bers, D.M., Shannon, T.R.  (2005)  Increased Sarcoplasmic Reticulum Calcium Leak in Heart Failure in Due to Ca-Calmodulin-Dependent Protein Kinase II.  Circulation 112:II277.

 

Curran, J.W., Guo, T., Bers, D.M. and Shannon, T.R. (2006) Simultaneous Measurements of Free Cytosolic and Free SR Ca Using Fura-2 and Fluo-5N to Assess SR Ca Leak in Cardiac Myocytes.  Biophys. J.  90:68a.

 

Launikonis, B.S., Zhou, J., Royer, L., Shannon, T.R., Rios, E. and Brum, G.  (2006)  A Ca transient Inside the Sarcoplasmic Reticulum Accompanies Ca2+ Release Induced by Low [Mg2+] in Frog Muscle Fibers.   Biophys. J. 90:68a.


Zhou, J., Launikonis, B.S., Royer, L., Shannon, T.R., Brum, G., Rios, E.  (2006)  Skraps of Ca2+ Depletion Suggest an Alternative Source for Ca2+ sparks and Global Ca2+ Release in Muscle.  Biophys. J. 90:68a.