Laboratory of Lothar A. Blatter - Department of Molecular Biophysics and Physiology, Rush University Chicago

Laboratory Members

Lothar A. Blatter, M.D.,, Professor
Jaime DeSantiago, M.D., Ph.D., Instructor
Giedrius Kanaporis, Ph.D., Assistant Professor
Stefanie Walther, M.D., Instructor


Laboratory of Lothar A. Blatter

Research Interest

Dr. Blatter's research group investigates control of intracellular calcium and its role in the regulation of excitation-contraction and excitation-transcription coupling in cells of the cardiovascular system.

Laboratory of Lothar A. Blatter

Cardiac Physiology. Study of mechanisms of excitation-contraction coupling and calcium regulation in cardiac (ventricular and atrial) muscle with the combined use of confocal imaging techniques and voltage clamp methods. Investigation of the regulation of cardiac ryanodine receptor calcium release channel incorporated into lipid bilayer. Investigation of the mechanism of cardiac alternans and cellular mechanisms of arrhythmias in cardiac hypertrophy and heart failure. Study of the role of IP3-dependent Ca signaling for excitation-contraction coupling, arrhythmias, and cardiac hypertrophy.

Study of nuclear Ca signaling and regulation of translocation of transcription factor NFAT. Investigation of redox regulation of SR Ca release. Study of NO-dependent signaling pathways in cardiac cells. Study of oxidative stress and cardiac energy metabolism and its effects on excitation-contraction coupling and Ca signaling. Study of intracellular pH and measurement of intracellular [Na] with fluorescence 2-photon confocal microscopy. Study of the mechanism of pacemaker activity in cardiac cells.

Vascular Physiology. Investigation of cellular and molecular mechanisms of [Ca]i regulation in vascular endothelial cells with high temporal and spatial resolution, using digital video fluorescence microscopy and laser scanning confocal microscopy. Study of the spatio-temporal regulation of capacitative calcium entry in vascular endothelial cells. Investigation of cellular mechanisms of the regulation of nitric oxide (NO) production and release from vascular endothelial cells. Study of the role of NO for [Ca]i regulation in vascular endothelial cells. Ca-dependent regulation of translocation of transcription factor NFAT in vascular endothelial cells.

Mitochondria. Study of the mechanisms governing mitochondrial membrane potential, mitochondrial ion channels, and the role of mitochondria for cellular calcium homeostasis. Study of mitochondrial NO synthase function and regulation. These studies involve optical measurements of membrane potential, pH, calcium and sodium in single isolated mitochondria as well as in mitochondria in permeabilized and intact cells. The Seahorse flux analyzer is used to investigate mitocondrial respiration and metabolism.

Research and Graduate Program at the Department of Molecular Biophysics & Physiology

For information on current research projects and graduate program.  View the Research and Graduate Program Brochure


Lothar A. Blatter, M.D.,
Dept. Molecular Biophysics & Physiology
Office: 1422 Jelke Bldg.; Laboratory: 1312-1316, 1401 Jelke Bldg.
1750 W. Harrison Street
Chicago, IL 60612
Phone: 312 563-3238
Fax: 312 942-8711
Curriculum Vitae

Job Opportunities

Research positions are available at the Postdoctoral and Assistant Professor levels.  View the Job Ad


NIH P01HL080101 (Blatter, Project leader): Ca and InsP3 receptor signaling in cardiac myocytes
NIH R01HL101235 (Blatter, multiple principal investigators): Mitochondrial dysfunction in cardiac hypertrophy and failure
Leducq Foundation Transatlantic Network (Blatter, Associate Member): Redox and nitrosative regulation of cardiac remodeling: novel therapeutic approaches for heart failure
NIH R01HL062231: E-C coupling and Ca2+ regulation in atrial myocytes (completed)


Laboratory of Lothar A. Blatter
Laboratory of Lothar A. Blatter

View publications from the Blatter laboratory on PubMed

Department of Molecular Biophysics & Physiology | Rush University | Medical Center

This page last modified February 20, 2015 © Rush University